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Cucurbit Genetics Cooperative Report 6:48 (article 24) 1983

Linkage of Red Stem and Male Sterile-1 in Muskmelon

J. D. McCreight

U. S. Department of Agriculture, Agricultural Research Service, P. O. Box 5098, Salinas, CA 93915

The seedling marker gene red stem (3) was crossed with male sterile-1 (1) to determine the linkage relationship between these two loci. Three F2 families segregated 559 green, fertile:195 green, male sterile:221 red stem, fertile:2 red stem, male sterile, X2=66.6316, which strongly suggested linkage between these loci. Eleven repulsion phase backcross (BC) progenies segregated in a poor fit to the expected 1:1 single factor and 1:1:1:1 dihybrid ratios (Table 1).

Table 1. Repulsion phase backcross segregation data for red stem and male sterile-1.


 

Phenotype


 

 

 

 

Normal


red stem


Single Factor X2


Linkage X2


 

fertile

male
sterile

fertile

male
sterile

  r

  ms-1

 


Deviation

484

990

1127

243

 3.8030

50.2405

711.5384

Heterogeneity

 

 

 

 

14.6701

42.1054

 


Two of the 11 BC progenies had a poor (P<0.05) fit to the expected 1:1 ratio for red stem. Seven progenies had a poor fit to the expected 1:1 ratio for male sterile-1. The BC, contingency table (2), linkage X2=711.5384 which strongly supported the F2 data. The recombination fraction, p (2) between these loci was 0.2556±0.0081.

Literature Cited

  1. Bohn, G. W. and T. W. Whitaker. 1949. A gene for male sterility in the muskmelon (Cucumis melo L.). Proc. Amer. Soc. Hort. Sci. 53:309–314.
  2. Mather, K. 1963. The measurement of linkage in heredity. John Wiley and Sons, New York. 149 pp.
  3. Mc Creight, J. D. and G. W. Bohn. 1979. Descriptions, genetics, and independent assortment of red stem and pale in muskmelon (Cucumis melo L.). J. Amer. Soc. Hort. Sci. 104:721–723.
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