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Cucurbit Genetics Cooperative Report 7:51-53 (article 23) 1984

Linkage Studies in Muskmelon

Pitrat, M.

I.N.R.A., Centre de Recherches Agronomiques d'Avignon, B.P. 94, 84140 Montfavet, France

Until now only 4 gene linkages have been described in muskmelon: (1) bush (b) and yellow virescent (yv) - recombination 19.4% (5); (2) virus aphid transmission resistance (Vat) and Flaccida necrosis (Fn) - recombination 11.6%; (3) red stem (r) and male sterile-1 (ms-1) - recombination 25.6% (1); and (4) Zucchini yellow mosaic virus resistance (Zym) and andromonoecious (a) - recombination 13.1% (3).

A program was begun to find linkage relationships between the following genes:

  1. Fusarium oxysporum f. sp. melonis resistance: Fom-1 (resistance to pathotypes 0 and 2) in 'Doublon' and Fom-2 (resistance to pathotypes 0 and 1) in PI 414723 (supplied by G.W. Bohn).
  2. Watermelon mosaic virus-1 resistance (Wmv) in PI 414723.
  3. Necrotic spot virus resistance (nsv) to muskmelon necrotic spot virus in VA 435 supplied by Virginia Truck Expt. Sta.
  4. Zucchini yellow mosaic virus resistance (Zym) in PI 414723.
  5. Virus aphid transmission resistance (Vat) in PI 414723 or PI 161375.
  6. Powdery mildew resistance-X (Pm-X) resistance to Sphaerotheca fuliginea race 2 in PI 414723 (exact powdery mildew resistance gene is under study).
  7. glabrous (gl) in Arizona gl B supplied by R.E. Foster.
  8. yellow green (yg) in Arizona gl B.
  9. red stem (r) in 30569 supplied by J. D. McCreight.
  10. bush (b) in 'Topmark Bush' supplied by F. W. Zink.

F2 data from 4 of 19 gene combinations among these 10 genes studied to date strongly indicated linkage. The recombination fractions for these 4 gene pairs were calculated using the maximum likelihood method (Table 1). F2 data indicated independent assortment between the other 15 gene pairs (Table 2).

Table 1. Linkage between 4 gene pairs in muskmelon.

Genes

Phasez

Number of plants observed in the expected dihybrid ratio

Chi square

Recombination

9

3

3

1

Value

Standard error

Fom-1

Wmv

R

104

52

44

0

19.876

0.0

7.07

Fom-2

yg

R

161

10

17

53

145.309

11.4

6.34

Zym

Pm-X

C

110

47

40

0

15.314

0.0

7.12

r

gl

R

367

106

128

8

27.745

30.9

3.61

zC=coupling; R=repulsion.

Table 2. Independent assortment between 15 gene pairs in muskmelon.

Genes

Phasez

Number of plants observed in the expected dihybrid ratio

Chi square

9

3

3

1

Fom-1

gl

R

159

61

51

19

1.163

Wmv

g

R

113

31

36

13

0.999

Vat

Zym

C

107

36

29

17

3.461

Wmv

nsv

R

111

32

45

12

2.347

gl

yg

C

1177

394

377

141

1.402

yg

b

R

138

41

49

19

1.594

yg

nsv

R

167

62

56

19

0.550

Pm-X

gl

R

151

54

44

7

6.486

Zym

nsv

R

96

40

35

18

4.834

Vat

b

R

100

38

32

11

0.638

Vat

nsv

R

105

48

32

14

4.444

b

gl

R

141

47

46

13

0.428

b

r

R

108

31

31

9

1.350

gl

nsv

R

178

50

50

24

3.330

Zym

b

R

83

25

23

12

1.796

zC=coupling; R=repulsion.

Vat was already found independent from Fom-1, Fom-2 and ms-1 (2), Zym independent from Fom-1 and Fom-2 (3) and Fom-l independent from Fom-2 (4). The muskmelon linkage groups may tentatively be summarized from these and published data:

  • Linkage group 1 : b - yv
  • Linkage group 2 : Vat - Fn
  • Linkage group 3 : ms-1 - r - gl
  • Linkage group 4 : Zym - a - Pm-X
  • Linkage group 5 : Fom-l - Wmv
  • Linkage group 6 : Fom-2 - yg.

Gene nsv is independent from linkage groups 2 to 6. Its independence with group 1 is under study. The order in groups 3 and 4 is still unknown.

Literature Cited

  1. McCreight, J.D. 1983. Linkage of red stem and male sterile-1 in muskmelon. Cucurbit Genetics Coop. Rept. 6:48.
  2. Pitrat, M. and H. Lecoq. 1982. Relations genetiques entre les resistances par non-acceptation et par antibiose du melon a Aphis gossypii. Recherches de liaisons avec d'autres genes. Agronomie 2:503-508.
  3. Pitrat, M. and H. Lecoq. 1984. Inheritance of zucchini yellow mosaic virus resistance in Cucumis melo L. Euphytica 33 (in press).
  4. Risser, G. 1973. Etude de l'heredite de la resistance du melon (Cucumis melo) aux races 1 et 2 de Fusariuma oxysporum f. sp. melonis. Ann. Amelior. Plantes 23:259-263.
  5. Zink, F.W. 1977. Linkage of virescent foliage and plant growth habit in muskmelon. J. Amer. Soc. Hort. Sci. 102:613-615.
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Page citation: Wehner, T.C., Cucurbit Genetics Cooperative;
Created by T.C. Wehner and T. Ng, 1 June 2005; design by C.T. Glenn;
send questions to T.C. Wehner; last revised on 30 November, 2009