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Cucurbit Genetics Cooperative Report 9:64-67 (article 19) 1986

Attempt at Localization of male sterile-4, cut leaf and virescent Mutants in Muskmelon

M. Pitrat, C. Ferriere and M. Ricard

INRA, Station d'Amelioration des Plantes Maraicheres, 84140 Montfavet, France

We have tried to find some linkages between male sterile-4 (ms-4) supplied by P. LOZANOV (Bulgaria) (2), cut leaf (cl) supplied by I. VELICH (Hungary) (5) or virescent (v) supplied by P.E. NUGENT (South Carolina, USA) (1) with other genes belonging to linkage groups already described (3, 4).

Male sterile-4 (ms-4) was found independent (Table 1) from genes belonging to linkage groups 1 to 7. Cut leaf (cl) was found independent (Table 2) from genes belonging to groups 1 to 7 and from ms-4. Virescent (v) has already been found independent from groups 2, 3, and 6 (4). We found it independent from genes belonging to groups 1, 4, and 7 and from ms-4 and cl (Table 3). It must be still tested with FOM-1 or Prv (synonym Wmv) from group 5.

Table 1. Segregation data observed in F2 progenies between male sterile -4 (ms-4) and si-1, Vat, gl, r, Zym, Fom-1, yg and nsv.

Linkage group
Genes
[Male fertile]
ms-4+ /-
[Male sterile]
ms-4/ms-4
chi square
Value
(9:3:3:1)
Probability
1
si-1+/-
si-1/si-1
121
31
4.516
21%
 
41
7
2
Vat/-
Vat+/Vat+
175
61
3.030
39%
 
55
12
3
gl+/-
gl/gl
244
75
1.171
76%
 
71
26
3
r+/-
r/r
125
43
2.008
57%
 
33
18
4
Zym/-
Zym+/Zyn+
111
43
6.213
11%
 
27
18
5
Fom-1/-
Fom-1+/Fom-1+
154
57
7.719
5%
 
38
25
6
yg+/-
yg/yg
117
32
1.333
72%
 
40
11
7
nsv+/-
nsv/nsv
226
83
5.751
12%
 
77
14

Table 2. Segregation data observed in F2 progenies between cut leaf (cl) mutant and si-1, Fn, Pa, Zym, Fom-1, Fom-2, ms-2, and ms-4.

Linkage group
Genes
[normal]
cl+/-
[cut leaf]
cl/cl
chi-square
Value
(9:3:3:1)
Probability
1
si-1+/-
si-1/si-1
164
50
5.446
14%
58
28
2
Fn/-
Fn+/Fn+
100
39
5.796
12%
50
11
3
Pa/Pa+
Pa+/Pa+
114
36
0.602z
90%
59
22
4
Zym/-

Zym+/Zym+

137
52
0.983
81%
50
14
5
Fom-2/-
Fom-2+/Fom-2+
110
38
0.249
97%
38
14
6
Fom-2/-
Fom-2+/Fom-2+
227
72
4.466
22%
66
34
6
ms-2+/-
ms-2/ms-2
107
37
0.236
97%
39
12
7
nsv+/-
nsv/nsv
165
52
3.218
36%
57
26
ms-4+/-
109
44
2.254
47%
ms-4/ms-4
32
15

z tested for 6:3:2:1 segregation because Pa/Pa is lethal.

Table 3. Segregation data observed among the normal green plants in F2 progenies segregating for virescent (v) and si-1, Zym, Fom-2, nsv, ms-4, and cl.

Linkage group
Genotypes
segregation among normal (v+/-) plants
chi-square ( 3 : 1 )
Value
Probability
1 si-1+/ : si-1/si-1
121
:
34
0.776
38%
4 Zym/- : Zym+ /Zym+
125
:
39
0.130
72%
6 Fom-2/- : Fom-2+/Fom-2+
229
:
70
0.402
53%
7 nsv+/- : nsv/nsv
141
:
47
0.428
93%
  ms-4+/- : ms-4/ms-4
127
:
34
1.294
26%
cl+/- : cl/cl
222
:
80
0.358
55%

Literature Cited

  1. Hoffman, J.C. and P.E. Nugent, 1973. Inheritance of a virescent mutant of muskmelon. J. Hered. 64:311-312.
  2. Locanov, P., 1983. Selekcija na mazkosterilini roditelski komponenti za ulesnjavana na proizvodstvoto na hibridni semena ot papesi. Dokl. na parva nauchna ikonferencija po genetika i selekapam Razgrad.
  3. Pitrat, M., 1984. Linkage studies in muskmelon. Cucurbit Genetics Coop. Rept. 7:51-53.
  4. Pitrat, M., 1985. Genetic linkages in muskmelon. Cucurbit Genetics Coop. Rept. 8:50-54.
  5. Velich, I. and I. Fulop, 1970. A new muskmelon type of cut leaf character. Zoldsegtermesztes 4:107-112.
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Page citation: Wehner, T.C., Cucurbit Genetics Cooperative;
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